Journal: Scientific Reports
Article Title: The proteasome inhibitor bortezomib attenuates renal fibrosis in mice via the suppression of TGF-β1
doi: 10.1038/s41598-017-13486-x
Figure Lengend Snippet: Cell cycle arrest and apoptosis induced by AA were ameliorated by BZM treatment. ( a ) Representative immunoblot of Bax, Bcl2, cyclin B1 and cyclin D1 in AAN mice kidneys treated with BZM. ( b ) Densitometry analysis of the proteins analyzed in ( a ). BZM significantly suppressed AA-induced increases in the expression of Bax, cleaved Bcl2, and ratio of cyclin B1 to cyclin D1. Values presented are means ± SEM. * P < 0.05; ** P < 0.01 (n = 4).
Article Snippet: The primary antibodies used in this study were rabbit anti-αSMA antibody (Abcam, Inc. Cambridge, UK), goat anti-KIM-1 antibody (R&D Systems, Inc. Minneapolis, MN), goat anti-NGAL antibody (R&D Systems, Inc. Minneapolis, MN), mouse anti-TGF-beta 1 antibody (R&D Systems, Inc. Minneapolis, MN), rabbit anti-Bax antibody (Abcam, Inc. Cambridge, UK), rabbit anti-Bcl2 (D17C4) antibody (Cell Signaling, Danvers, MA), rabbit anti-phosphorylated Smad3 antibody (Cell Signaling, Danvers, MA), rabbit anti-Smad2/3 antibody (Cell Signaling, Danvers, MA) , , , rabbit anti-NF-kappa B p65 (D14E12) XP antibody (Cell Signaling, Danvers, MA), rabbit anti-cyclin B1 antibody 4138 (Cell Signaling, Danvers, MA), rabbit anti-cyclin D1 antibody 2978 (Cell Signaling, Danvers, MA), rabbit anti-GAPDH antibody (Cell Signaling, Danvers, MA), and rabbit anti-Histone H3 antibody (Abcam, Inc. Cambridge, UK).
Techniques: Western Blot, Expressing